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Determining safe pH for your protein

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PCB Cafe :: Expertise Search :: Technical Q&A's

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Determining safe pH for your protein Empty Determining safe pH for your protein

Post by SarahA Thu May 19, 2022 3:51 pm

Hey All,

I'm trying to do a bunch of assays with an acyl carrier protein, and want to know its stability at different pHs. What's the best way to do this? Normally with an enzyme I'd just see if its still active, but holo-ACP doesn't have activity per se. Estimated pI of the protein is 7.23 if that helps!

SarahA
Narayan Lab

Posts : 1
Join date : 2022-05-19

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Determining safe pH for your protein Empty Re: Determining safe pH for your protein

Post by Olive Fri May 20, 2022 7:20 pm

Hey Sarah, not too familiar with the conserved residues of ACP, but does it have any cysteine residues? How about tryptophans?
Olive
Olive
Stockbridge Lab

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Join date : 2021-03-29
Age : 23

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